Generally, no . Transferring data from the PLC (uploading) does not interrupt the running process. It is a non-disruptive read operation. However, performing a restore (writing to the PLC) will typically stop the CPU to overwrite the program and configuration and may cause downtime.
In today's highly automated manufacturing environment, a Programmable Logic Controller (PLC) is the brain behind every critical operation. These industrial computers are embedded with custom logic, complex timers, and sequential instructions that control everything from assembly line robots to entire chemical processing plants. If that logic is lost, the consequences are immediate and devastating—production lines grind to a halt, causing losses that can skyrocket into hundreds of thousands of dollars per hour. Plc Backup Tools V6 0 13