Primer3 0.4.0 — Link

Ensure there are no ambiguous bases (e.g., Ns) in the input sequence.

Window: Expand PRIMER_MIN_TM and PRIMER_MAX_TM . A narrow window often disqualifies otherwise functional primers. primer3 0.4.0

: It operates with a minimal memory footprint, making it ideal for high-throughput batch processing and integration into lightweight Linux containers. Ensure there are no ambiguous bases (e